Question

How can we observe Rhizobium bacteria in the roots of leguminous plant? Select the correct answer from above options Science questions and answers, Science questions pdf, Science question bank, Science questions and answers pdf, mcq on Science pdf,Science book pdf,Science pdf notes,Science lecture notes,Science of light,maxwell Science,Science proposed by,electromagnetic theory engineering physics,Science nptel

Answer 1

Take a plantlet of fenugreek, groundnut or any other bean and sterilize it with a 3 to 5% solution of hydrogen peroxide. Afterwards, keep it in a 70% solution of ethyl alcohol for 4 to 5 minutes. Clean the roots with sterile water and take thin sections of the root nodules. Select a good section and place it an a solution of safranin for 2 to 3 minutes. Place the stained section on a glass slide, cover it with a coverslip and observe it under the compound microscope. The pinkish rod shaped organisms are the Rhizobium bacteria.